Supplementary Figure 9: STED power did not induce magnetic bead displacement.

From left to right: bright-field images of the cell, GFP-Histone 2B fluorescence images, displacement map images and enlarged displacement maps. Nucleus is outlined with a dashed line. The thick white arrows point to the magnetized bead; the pink dot indicates the magnitude and direction of the bead displacement (<5 nm, suggesting no additional displacements by STED); the color bar indicates the displacement magnitude. (a) No applied stress in confocal images. The FWHM is 236 nm; (b) the same nucleus in 90% STED power (~64.8 mW) images. The FWHM is 120 nm. Image acquisition time is 320 ms per image. Scale bars, 5 μm.