Supplementary Figure 10: System description of 3D-MTC with confocal fluorescence microscopy.

The platform of interfacing 3D-Magnetic Twisting Cytometry (3D-MTC) with the confocal fluorescence microscopy (with an option of stimulated emission depletion (STED) nanoscopy) includes three parts: magnetic field generator subsystem, monitoring and analysis subsystem, and observing subsystem. Each subsystem includes hardware equipment, interface board, control interface program or analysis modules.