Supplementary Figure 4: Acquired Image window of the Olympus FluoView program.
From: Measurement of drug-target engagement in live cells by two-photon fluorescence anisotropy imaging
The two images (red boxes 1,2) show simultaneous acquisition of fluorescence emission at two orthogonal state of polarization as detected at PMT3 and PMT4, for a solution of fluorescein diluted in a mixture of water-glycerol (w/w) at 95%. Image of FLUOVIEW program adapted with permission from Olympus.
