Supplementary Figure 3: HR-MS and RP-HPLC analysis of biotin-6-ahx-DTyr(tBu)-Tic-Ser(tBu)-COOH.
From: Synthesis of a HyCoSuL peptide substrate library to dissect protease substrate specificity
Peptide was synthesized according to the strategy presented in the main protocol (Block A, steps 100-124), and used without further purification. The purity of the peptide was confirmed using analytical HPLC (UV detector, C8 column, 220nm). The molecular mass of the peptide was confirmed using High Resolution Mass Spectrometer WATERS LCT premier XE with Electrospray Ionization (ESI) and Time of Flight (TOF) module.
