Supplementary Figure 1: HyCoSuL synthesis and quality control exemplified with the P2 sublibrary.
From: Synthesis of a HyCoSuL peptide substrate library to dissect protease substrate specificity
P2 HyCoSuL sublibrary contains amino acids mixtures at P3 and P4 positions. To test whether the coupling of isokinetic mixture provided the equal distribution of amino acids in P3 (and P4) position an Edman degradation can be utilized. After coupling and de-protection of P3 position (steps 38-44) several beads are subjected for the analysis in order to determine the molar distribution of amino acids at the N-terminal end of a peptide. The same procedure can be applied to test the equimolar coupling of amino acids mixture to the P4 position.
