Supplementary Figure 2: Microscopic features of cells, free nuclei, and free organelles in a homogenate. | Nature Protocols

Supplementary Figure 2: Microscopic features of cells, free nuclei, and free organelles in a homogenate.

From: Rapid immunopurification of mitochondria for metabolite profiling and absolute quantification of matrix metabolites

Supplementary Figure 2

Representative micrographs of a homogenate of HeLa cells expressing the HA-MITO construct. Cells were homogenized using 25 strokes without rotation of the plunger and a sample of the homogenate was diluted with KPBS and then transferred to the well of a 6 well plate for microscopy. The physical features (gray) and EGFP signals (green) of the components of the homogenate were assessed. TL, standard transmitted light microscopy; C, cells; N, free nuclei; O, free organelles. Scale bar, 10 μm. For this figure, HeLa cells were cultured in complete DMEM, authenticated by the Duke University DNA Analysis Facility, and tested for mycoplasma contamination.

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