Supplementary Figure 1: KTR translocation in different cell lines. | Nature Protocols

Supplementary Figure 1: KTR translocation in different cell lines.

From: Live-cell measurements of kinase activity in single cells using translocation reporters

Supplementary Figure 1

Time relative to stimulation is shown on each images.

(A) HeLa cells expressing JNK KTR were stimulated with anisomycin 50 ng/ml.

(B) HEK293 cells expressing JNK KTR were stimulated with anisomycin 50 ng/ml.

(C) Raw 264.7 cells expressing JNK KTR were stimulated with LPS 100 ng/ml.

(D) PC-12 cells expressing ERK KTR were stimulated with NGF 25 ng/ml.

(E) Primary Bone Marrow-derived Macrophages (BMDMs) isolated from mice expressing JNK KTR were stimulated with LPS 100 ng/ml.

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