Supplementary Figure 3: Gating strategy for analyzing human engraftment in NSG mice.
From: CRISPR/Cas9 genome editing in human hematopoietic stem cells
Representative FACS plots show the gating strategy upstream of the FACS plots shown in Figure 4D. Cells are identified in a forward/side scatter plot (FCS-A and SSC-A) and single cells discriminated from doublets using SSC-W/SSC-H and FSC-W/FSC-H plots. Live cells and murine red blood cells (RBCs) are discriminated based on propidium iodide and an anti-murine Ter119 antibody. Out of the live non-RBCs, human cells can be gated based on huCD45 and HLA-ABC expression as depicted in Figure 4D. Adapted with permission from ref. 15, Springer Nature.
