Supplementary Figure 5: Schema of dissection of V-SVZ tissue. | Nature Protocols

Supplementary Figure 5: Schema of dissection of V-SVZ tissue.

From: FACS isolation of endothelial cells and pericytes from mouse brain microregions

Supplementary Figure 5

Overview of V-SVZ dissection. Steps refer to numbers in Box 2. To dissect the V-SVZ from the brain, make 4 coronal cuts through the brain and discard the most rostral and caudal sections. From each coronal section, dissect the V-SVZ (thin brown wedge beside the lateral ventricle) by cutting ventrally below the ventricle (step 3), dorsally by pulling up on the corpus callosum (step 4), and laterally to remove striatum (step 5). Repeat on the contralateral side before proceeding to the next slice.

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