Supplementary Figure 2: CPM and effective volume dependency on pinhole diameter and laser power | Nature Protocols

Supplementary Figure 2: CPM and effective volume dependency on pinhole diameter and laser power

From: Quantitative mapping of fluorescently tagged cellular proteins using FCS-calibrated four-dimensional imaging

Supplementary Figure 2

(a) Counts per molecule (CPM) as function of the pinhole diameter for Alexa488 in water. (b) Effective confocal focal volume estimated as described in the supplement (Supplementary Notes 1–2 and Box 2) as function of the pinhole diameter for Alexa488. (c) Effective confocal focal volume as function of the excitation laser (Argon 488 nm). The pinhole was 34 μm.

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