Supplementary Figure 2: Scheme of expected junction PCR result.
From: Generation and validation of homozygous fluorescent knock-in cells using CRISPR–Cas9 genome editing
A forward primer binding at the 5′ end outside of the left homology arm and a reverse primer binding to the fluorescent marker gene will result in one PCR product of the expected size. To test if all alleles are tagged with the fluorescent marker at the correct locus, a forward primer located 5′ outside of the left homology arm and a reverse primer 3′outside of the right homology arm were used. Two PCR products using this primer set will indicate heterozygote clones and one PCR product which runs at the expected size of the tagged gene will indicate homozygosity.
