Supplementary Figure 3: Southern blot transfer of DNA.
From: Generation and validation of homozygous fluorescent knock-in cells using CRISPR–Cas9 genome editing
This scheme depicts how to build up the southern blot transfer as described in step 48| Option B, step xii-xxiii). Two long filter papers are dipped into a tank filled with 10X SSC and used as wicks. Gel, nylon membrane and filter papers were sandwiched on a glass plate as depicted to transfer the DNA from the gel onto the membrane.
