Supplementary Figure 4: Example sequences containing point mutations.
From: Generation and validation of homozygous fluorescent knock-in cells using CRISPR–Cas9 genome editing
Sanger sequencing was performed with a PCR fragment (lower sequence line #1-278) which was aligned to the expected sequence of the GOI (middle sequence line labeled with #3201-3500). The overlaying green and red lines indicate a point mutation at position 3411 of the expected sequence, i.e. one allele has the nucleotide T at this position (green line) whereas another allele has the nucleotide A (red line) at the same position. This indicates a point mutation (red A) within one of the alleles.
