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Indirect activation

Nature Reviews Cancer volume 9, page 75 (2009)Cite this article

Most tyrosine kinases are tyrosine phosphorylated after activation, enabling Todd Golub and colleagues to use this phosphorylation status as a marker of activation. They coupled tyrosine kinase-specific antibodies to polystyrene beads, allowing affinity purification of tyrosine kinases from cancer cell lines or tumour samples. An anti-phosphotyrosine antibody was then used to detect the bead complexes that contained activated kinases by flow cytometry.

The authors profiled 130 human cancer lines, creating a tyrosine kinase activation catalogue. They validated their method by detecting the expected patterns of tyrosine phosphorylation in particular cancer cell types, such as ABL1 phosphorylation in chronic myeloid leukaemia cells containing a BCR–ABL1 translocation.

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ORIGINAL RESEARCH PAPER

  1. Du, J. et al. Bead-based profiling of tyrosine kinase phosphorylation identifies SRC as a potential target for glioblastoma therapy. Nature Biotech. 21 Dec 2008 (doi: 10.1038/nbt.1513)

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Authors
  1. Meera Swami
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Swami, M. Indirect activation. Nat Rev Cancer 9, 75 (2009). https://doi.org/10.1038/nrc2592

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