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Mouse models

Novel in vivo RNAi screen in mice

Nature Reviews Cancer volume 13, page 683 (2013)Cite this article

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Beronja et al. previously developed an ultrasound-guided in utero lentiviral transduction method to target embryonic skin epithelium in vivo with pools of lentiviral short hairpin RNAs (shRNAs). In this new study, the authors extend the above method to conduct parallel genome-wide screens of 78,000 shRNAs for genes required for normal skin growth in wild-type embryos, and genes required for hyperplastic skin growth in embryos that express oncogenic Hras in skin cells. They successfully identified ∼1,800 genes required for normal growth and 160 genes required for hyperplastic growth.

The top two candidates that emerged from their screen were β-catenin and mixed-lineage leukaemia translocated to 6 gene (Mllt6). Deregulation of β-catenin is known to be involved in several cancer types, including skin cancer, whereas Mllt6 has only previously been implicated in the development of leukaemias.

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References

  1. Beronja, S. et al. RNAi screens in mice identify physiological regulators of oncogenic growth. Nature http://dx.doi.org/10.1038/nature12464 (2013)

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Authors
  1. Isabel Lokody
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Lokody, I. Novel in vivo RNAi screen in mice. Nat Rev Cancer 13, 683 (2013). https://doi.org/10.1038/nrc3604

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