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Cloning

Taking technical and ethical hurdles

Nature Reviews Molecular Cell Biology volume 8, page 516 (2007)Cite this article

It has long been believed in the cloning field that successful somatic-cell nuclear transfer (SCNT) — a way to make ES-cell lines — requires the use of unfertilized eggs. However, this study reports a solution that overcomes this problem and shows that fertilized eggs can also be used, at least in mice. The authors used condensed chromosomes from nocodazole-arrested donor zygotes and two- and eight-celled embryos. The chromosomes were injected into the cytoplasm of treated recipient zygotes, and the resulting blastocysts were returned to foster mothers. Donors from all three stages of development led to some live births.

...describe a different approach for the production of ES-cell lines.

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References

ORIGINAL RESEARCH PAPER

  1. Egli, D. et al. Developmental reprogramming after chromosome transfer into mitotic mouse zygotes. Nature 447, 679–685 (2007)

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FURTHER READING

  1. Colman, A. & Burley, J. Recycling the abnormal. Nature 447, 649–650 (2007)

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Authors
  1. Ekat Kritikou
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Kritikou, E. Taking technical and ethical hurdles. Nat Rev Mol Cell Biol 8, 516 (2007). https://doi.org/10.1038/nrm2212

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