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Cell cycle

Destruct and arrest

Nature Reviews Molecular Cell Biology volume 10, page 7 (2009)Cite this article

Using nocodazole (a microtubule-depolymerizing agent) to activate the SAC in live-imaging studies of human cells, Nilsson et al. observed that a fluorescent CDC20 fusion protein was degraded in response to SAC. Degradation of CDC20 started at the time of nuclear envelope breakdown, which coincides with the time when APC/C recognizes its early mitotic substrates. This implies that CDC20 might be a target of APC/C. Indeed, the use of small interfering RNAs (siRNAs) against APC/C or the SAC protein MAD2 blocked CDC20 degradation, which suggests that CDC20 is degraded by APC/C in response to the SAC.

Size-exclusion chromatography and quantitative immunoblotting experiments in nocodazole-treated cells revealed that CDC20 primarily interacts with the SAC component BUBR1 and not with MAD2, as previously thought.

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References

ORIGINAL RESEARCH PAPERS

  1. Nilsson, J. et al. The APC/C maintains the spindle assembly checkpoint by targeting Cdc20 for destruction. Nature Cell Biol. 10, 1411–1420 (2008)

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  2. Kimata, Y. et al. A role for the Fizzy/Cdc20 family of proteins in activation of the APC/C distinct from substrate recruitment. Mol. Cell 32, 576–583 (2008)

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FURTHER READING

  1. Peters, J.-M. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nature Rev. Mol. Cell Biol. 7, 644–656 (2006)

    Article  CAS  Google Scholar 

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Authors
  1. Francesca Cesari
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Cesari, F. Destruct and arrest. Nat Rev Mol Cell Biol 10, 7 (2009). https://doi.org/10.1038/nrm2612

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