Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Research Highlight
  • Published:

Membrane dynamics

How lysosomes SNARE autophagosomes

Nature Reviews Molecular Cell Biology volume 14, page 65 (2013)Cite this article

Subjects

Access through your institution
Buy or subscribe

In mouse embryonic fibroblasts (MEFs), STX17 colocalized with the autophagosome marker LC3 under starvation conditions (which are known to induce autophagy) but not in resting cells. A truncation mutant of STX17 lacking the amino-terminal SNARE domain also colocalized with LC3 in starved cells, which shows that the autophagosomal localization of STX17 is not a result of transfer from another organelle during SNARE-mediated membrane fusion.

Knockdown of STX17 resulted in the accumulation of LC3+ autophagosomes in human HeLa cells even under normal growth conditions. These LC3+ autophagosomes did not colocalize with late endosomal or lysosomal markers and did not show any features of cytoplasmic degradation, which suggests that fusion between autophagosomes and late endosomes and/or lysosomes is blocked in the absence of STX17.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Access through your institution

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

ORIGINAL RESEARCH PAPER

  1. Itakura, E. et al. The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes. Cell 151, 1256–1269 (2012)

    Article  CAS  Google Scholar 

Download references

Authors
  1. Kirsty Minton
    View author publications

    Search author on:PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Minton, K. How lysosomes SNARE autophagosomes. Nat Rev Mol Cell Biol 14, 65 (2013). https://doi.org/10.1038/nrm3506

Download citation

  • Published:

  • Issue date:

  • DOI: https://doi.org/10.1038/nrm3506

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing