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Cell cycle

Why phosphorylation matters

Nature Reviews Molecular Cell Biology volume 3, page 153 (2002)Cite this article

One of the puzzles in S-phase biology is that although phosphorylation of replication proteins is generally believed to activate DNA replication, finding the biological effects of phosphorylation has been difficult to prove. But, reporting in Nature, Araki and colleagues provide several lines of evidence in budding yeast that the replication protein Sld2 is phosphorylated in a cyclin-dependant kinase (CDK) manner and that this is essential for chromosomal DNA replication.

The authors looked at Sld2 — which is required for an early step of chromosomal DNA replication — as it has six preferred CDK phosphorylation motifs, and mutations in any one of these motifs are lethal.

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References

ORIGINAL RESEARCH PAPER

  1. Masumoto, H. et al. S-Cdk-dependant phosphorylation of Sld2 essential for chromosomal DNA replication in budding yeast. Nature 415 , 651 –655 (2002)

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FURTHER READING

  1. Kamimura, Y. et al. Sld2, which interacts with Dpb11 in Saccharomyces cerivisiae, is required for chromosomal DNA replication. Mol. Cell. Biol. 18, 6102–6109 (1998)

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  2. Kelly, T. J. & Brown, G. W. Regulation of chromosome replication. Annu. Rev. Biochem. 69, 829–880 (2000)

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Authors
  1. Simon Frantz
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Frantz, S. Why phosphorylation matters. Nat Rev Mol Cell Biol 3, 153 (2002). https://doi.org/10.1038/nrm756

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