Figure 2: Genetic diversity of CRESS-DNA viruses.

The replication-associated protein (Rep) sequences of 659 circular Rep-encoding single-stranded DNA (ssDNA) viruses (CRESS-DNA viruses) were compared with 10 representative Repsequences from viruses classified in the families Geminiviridae, Nanoviridae, Circoviridae and Genomoviridae, and a group of alpha satellites that are associated with geminiviruses or nanoviruses. Amino acid sequences were aligned using Multiple Alignment using Fast Fourier Transform (MAFFT; G-INS-i option)70, and a maximum likelihood phylogenetic tree was constructed using Fasttree71. Branches with less than 50% SH (Shimodaira–Hasegawa)-like support were collapsed.