Supplementary Figure 1: Characterization of the GlyRS-NEDD8 interaction.
From: Neddylation requires glycyl-tRNA synthetase to protect activated E2
(a) Hydrogen-deuterium exchange (HDX) analysis of GlyRS in the presence of NEDD8. Changes in deuterium incorporation resulting from the NEDD8 interaction are mapped to the sequence and the crystal structure of GlyRS (PDB 2PME). (b,c) Di-glycine motif of NEDD8 is not essential for GlyRS. Biolayer interferometry analysis comparing GlyRS binding to the full-length NEDD8 (b) and the truncated NEDD8 lacking the C-terminal di-glycine motif (NEDD8ΔGG) (c). His-tagged NEDD8 proteins were immobilized to the Ni-NTA sensor tips. Tag-free GlyRS (yellow green, 250 nM; orange,500 nM) were used to detect the interaction with NEDD8.
