Supplementary Figure 2: GlyRS does not affect Ube2F conjugation. | Nature Structural & Molecular Biology

Supplementary Figure 2: GlyRS does not affect Ube2F conjugation.

From: Neddylation requires glycyl-tRNA synthetase to protect activated E2

Supplementary Figure 2

(a) Western blot analysis to detect N8-conjugation of Ube2F in HeLa cells transfected with plasmids expressing shRNAs against GlyRS (shGlyRS) or SerRS (shSerRS) or a control vector. (b) Biolayer interferometry analysis to compare the binding of Ubc12 and Ube2F (both at 1.0 µM) to immobilized GST-GlyRS.

Back to article page