Abstract
Background
Alterations of B2 adrenergic receptor (β2AR) can modulate the severity of asthma and the response to treatment. Therefore, we aimed to evaluate β2AR gene polymorphism at codons 16 and 27 and their effect on asthma severity and response to treatment in asthmatic children.
Methods
Case–control study was conducted on 156 children; 104 of them had bronchial asthma and 52 were healthy children (control group). Subjects of the study underwent history taking, clinical examination, pulmonary function tests, serum IgE level assessment, and identification of β2AR-16 A46G and β2AR-27 C79G polymorphism using PCR-Restriction Fragment length polymorphisms (RFLP) test.
Results
There was a higher frequency of Arg–Gly genotypes (odds ratio (OR)=6.57; confidence interval (CI): 2.42–18.81, P<0.001) and lower frequency of Arg–Arg (OR=4.7; CI: 2.05–10.95, P<0.001) among asthmatic children compared with that among controls at codon 16. The presence or absence of Gly16 or Glu27 either homozygous or heterozygous for both correlated with the grade of asthma severity. The presence of heterozygous Arg–Gly and Gln–Glu gives a better response to drug therapy than the presence of Gly–Gly and Glu–Glu genotypes at codons 16 and 27.
Conclusion
Polymorphism of β2AR at codons 16 and 27 correlates with asthma severity and response to treatment in asthmatic children.
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Alghobashy, A., Elsharawy, S., Alkholy, U. et al. B2 adrenergic receptor gene polymorphism effect on childhood asthma severity and response to treatment. Pediatr Res 83, 597–605 (2018). https://doi.org/10.1038/pr.2017.304
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DOI: https://doi.org/10.1038/pr.2017.304
