Fig. 6: Prestin activity in wild-type or mutant MYH1-expressing cells.
From: MYH1 deficiency disrupts outer hair cell electromotility, resulting in hearing loss
a Voltage-normalized nonlinear capacitance (NLC) relationship of overexpressed prestin with or without MYH1 in HEK293T cells. b Vh values measured in cells overexpressing prestin with or without MYH1. c Normalized charge transfer density measured in cells overexpressing prestin with or without MYH1. d Immunoprecipitation (IP) and coimmunoprecipitation (co-IP) of prestin- and MYH1-transfected HEK293T cells. The input lanes represent 1/10 of the immunoprecipitated cell lysate. e NLC relationship between overexpressed prestin and mutant MYH1. f Vh values and normalized charge transfer density measured from cells overexpressing prestin and mutant MYH1. g Normalized charge transfer density measured from cells overexpressing prestin and mutant MYH1. The data are presented as the means ± SEMs. Statistical comparisons were performed using two-sample independent t-tests in (b) and (c). *P < 0.05 and ****P < 0.0001.
