Table 1 List of Cas9 variants and orthologous Cas enzymes.
From: CRISPR-based functional genomics tools in vertebrate models
Cas9 variant | Source/mutations | PAM sequence | Reference |
|---|---|---|---|
SpCas9 (wild type) | Streptococcus pyrogens | NGG | |
SpCas9-NG | R1335V/L1111R/D1135V/G1218R/E1219F/A1322R/R1333P/R1335Q | NG (reduced activity at non-NGG) | |
VQR-SpCas9 | D1135V/R1335Q/T1337R | NGAN > NGNG > NGCG | |
VRER-SpCas9 | D1135V/G1218R/R1335E/T1337R | NGCG | |
xCas9 | A262T/R324L/S409I/E480K/E543D/M694I/E1219V | NGN (broad recognition) | |
SpG-Cas9 | D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R | NGN (prefers NGG/NGA/NGT) | |
SpRY-Cas9 | D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R/T1642I/Y1669A | NRN (R = A or G) | |
SpCas9-NRRH | D1135V/R1335P/T1337R/T1642R/A1722R/S1738R | NRRH (R = A or G; H = A, C, or T) | |
SpCas9-NRTH | D1135N/R1335P/T1337R/T1642R/A1722R/S1738R | NRTH (R = A or G; H = A, C, or T) | |
SpCas9-VRQR | D1135V/R1335Q/T1337R/D1356R | NGA | |
SpCas9-NAG | D1135E | NAG | |
SaCas9 | Staphylococcus aureus | NNGRRT | |
SaCas9-KKH | Staphylococcus aureus | NNNRRT | |
ScCas9 | Streptococcus canis | NNG | |
FnCas9 | Francisella novicida | YG (Y = C or T) | |
St1Cas9 | Streptococcus thermophilus | NNAGAAW | |
NmeCas9 | Neisseria meningitidis | NNNNGATT | |
Nme2Cas9 | Neisseria meningitidis | NNNNCC | |
eNme2Cas9 | Neisseria meningitidis (evolved) | NNNNTN or NNNNCN | |
CjCas9 | Campylobacter jejuni | NNNNRYAC | |
GeoCas9 | Geobacillus stearothermophilus | NNNNCRAA | |
SauriCas9 | Staphylococcus auricularis | NNGG | |
iSpymac-Cas9 | Streptococcus macacae and Streptococcus pyogens chimeric | NAAN | |
LbaCas12a | Lachnospiraceae bacterium | 5′-TTTN | |
AsCas12a (Cpf1) | Acidaminococcus sp. | 5′-TTTV (V = A, C, or G) | |
AsCpf-RR and AsCpf-RVR | Acidaminococcus sp. S542R/K607R and S542R/K548V/N552R | 5′-TYCV, 5′-TATV (V = A, C, or G; Y = C or T) | |
enAsCas12a | Acidaminococcus sp. | 5′-TTN | |
FnCas12a | Francisella novicida | 5′-TTN | |
AaCas12b | Alicyclobacillus acidoterrestris | 5′-TTTN | |
DpbCas12e (CasX) | Deltaproteobacteria | 5′-TTCN | |
Un1Cas12f1 | Uncultured organism | 5′-TTTR (R = A or G) |
