Table 2 List of base editors.
From: CRISPR-based functional genomics tools in vertebrate models
Base editor | Deaminase enzyme | Cas enzyme | Editing window (nt) | Reference |
|---|---|---|---|---|
CBEs | ||||
BE3 | rAPOBEC1 | nCas9-H840A | 4–8 | |
BE4max | rAPOBEC1 | nCas9-D10A | 4–8 | |
AncBE4max | Ancestral APOBEC1 | nCas9-D10A | 4–8 | |
SaBE4 | rAPOBEC1 | nSaCas9-D10A | 3–12 | |
BE3-Gam | rAPOBEC1 | nCas9-D10A | 4–8 | |
SaBE3-Gam | rAPOBEC1 | nSaCas9-D10A | 3–12 | |
BE4-Gam | rAPOBEC1 | nCas9-D10A | 4–8 | |
SaBE4-Gam | rAPOBEC1 | nSaCas9-D10A | 3–12 | |
CDA1-BE3 | PmCDA1 | nCas9-D10A | 4–8 | |
AID-BE3 | hAID | nCas9-D10A | 4–8 | |
evoAPOBEC1-BE4max | rAPOBEC1 | nCas9(D10A | 3–8 | |
evoFERNY-BE4max | FERNY* | nCas9-D10A | 1–8 | |
evoCDA1-BE4max | PmCDA1 | nCas9-D10A | 1–13 | |
SaBE3 | rAPOBEC1 | nSaCas9-H840A | 3–12 | |
SaKKH-BE3 | rAPOBEC1 | nSaKKHCas9-H840A | 3–12 | |
YEE-BE3 | rAPOBEC1-YEE | nCas9-H840A | 5–6 | |
EE-BE3 | rAPOBEC1-EE | nCas9-H840A | 5–6 | |
Target-AID | PmCDA1 | dCas9- D10A, H840A | 3–7 | |
Target-AID-NG | PmCDA1 | nCas9-D10A-NG | 2–8 | |
AIDx | hAIDx | dCas9-D10A, H840A | 5–9 | |
dCas9-AIDx | hAIDx | dCas9- D10A, H840A | 5–9 | |
HF-BE3 | rAPOBEC1 | HF1-nCas9-H840A | 4–8 | |
xBE3 | rAPOBEC1 | nxCas9-D10A | 4–8 | |
BE-PLUS | rAPOBEC11-scFV | nCas9-D10A | 4–16 | |
iSpy-macnCas9-BE3 | rAPOBEC1 | iSpy-macnCas9-D10A | 4–8 | |
R33A-BE4 | rAPOBEC1-R33A | nCas9-D10A | 5–7 | |
R33A/K34A-BE4 | rAPOBEC1-R33A/K34A | nCas9-D10A | 5–6 | |
BE3- R33A | rAPOBEC1-R33A | nCas9-D10A | 5–7 | |
BE3-R33A/K34A | rAPOBEC1-R33A/K34A | nCas9-D10A | 5–6 | |
eA3A-BE3 | Evolved hAPOBEC3G-N57G | nCas9-D10A | 4–8 | |
hA3A-BE3 | Evolved hAPOBEC3A | nCas9-D10A | 2–13 | |
SpRY CBE4max | rAPOBEC1 | SpRY-nCas9-D10A | 3–9 | |
SpG CBE4max | rAPOBEC1 | SpG-nCas9-D10A | 3–9 | |
nCDA1-BE3 | PmCDA1Δ | nCas9-D10A | 3–4 | |
Tad-CBE | Evolved TadA | nCas9-D10A | 3–8 | |
Td-CBEs | Evolved TadA | nCas9-D10A | 6–7 | |
eTd-CBE | Evolved TadA | nCas9-D10A | 5–6 | |
zevoCDA1-BE4max | Evolved CDA1 | nCas9-D10A | 1–9 | |
zevoCDA1-NL | Evolved CDA1 | nCas9-D10A | 1–7 | |
zevoCDA1-198 | Evolved CDA1 | nCas9-D10A | 1–5 | |
zevoCDA1-SpRY-BE4max | Evolved CDA1 | SpRY-nCas9-D10A | 1–9 | |
Adenine base editors | ||||
ABE7.10 | TadA-TadA* | nCas9 (D10A) | 4–7 | |
xABE | TadA-TadA* | x-nCas9 (D10A) | 4–7 | |
ABEmax | TadA-TadA* | nCas9 (D10A) | 4–7 | |
NG-ABEmax | TadA-TadA* | nCas9-NG (D10A) | 4–8 | |
ABEmaxAW | TadA-E59A-TadA*-V106W | nCas9 (D10A) | 4–8 | |
VQR-ABEmax | TadA-TadA* | VQR-nCas9 (D10A) | 4–8 | |
VRER-ABEmax | TadA-TadA* | VRER-nCas9 (D10A) | 4–8 | |
VRQR-ABEmax | TadA-TadA* | VRQR-nCas9 (D10A) | 4–8 | |
Sa-ABEmax | TadA-TadA* | Sa-nCas9 (D10A) | 4–14 | |
SaKKH-ABEmax | TadA-TadA* | SaKKH-nCas9 (D10A) | 4–14 | |
miniABEmax | TadA* | nCas9 (D10A) | 4–7 | |
ABE7.10 F148A | TadA-F148A-TadA*-F148A | nCas9 (D10A) | 5 | |
ScCas9-ABE (7.10) | TadA-TadA* | Sc-nCas9 (D10A) | 4–7 | |
SECURE-ABEs | TadA*-K20A/R21A or TadA*-V82G | nCas9 (D10A) | 4–7 | |
ABE8e | TadA* | nCas9 (D10A) | 4–7 | |
ABE8s | TadA* | nCas9 (D10A) | 3–10 | |
ABE9 | TadA* | nCas9 (D10A) | 5–6 | |
ABE10 | Artificial intelligence-designed TadA | nCas9 (D10A) | 5–7 | |
ABE-Ultramax | TadA* | nCas9 (D10A) | 3–9 | |
ABE-Umax-ex1 | TadA* | nCas9 (D10A) | 4–12 | |
ABE-Umax-ex2 | TadA* | nCas9 (D10A) | 8–16 | |
ABE-Umax-rest1 | TadA* | nCas9 (D10A) | 5–6 | |
ABE-Umax-ex1-rest1 | TadA* | nCas9 (D10A) | 4–6 | |
ABE-Umax-ex2-rest1 | TadA* | nCas9 (D10A) | 12–15 | |
