Fig. 2

PRKD1 E710D hotspot mutations and PRKD1/2/3 rearrangements in the histologic spectrum of polymorphous adenocarcinoma of the salivary gland. a Representative Sanger sequencing electropherograms of the PRKD1 E710D hotspot locus in polymorphous adenocarcinomas (PACs), cribriform adenocarcinomas of the salivary gland (CASGs), tumors with indeterminate features between PACs and CASGs (TIF) and tumors with predominant papillary pattern (TPPP). Arrows point to the altered base. b Representative micrographs of the fluorescence in situ hybridization (FISH) analysis of PRKD3 and PRKD1 in a PAC and a CASG using dual-color break-apart probes (red, 5′ probe; green, 3′ probe). c Frequency of PRKD1 E710D hotspot mutations and PRKD1/2/3 rearrangements in polymorphous adenocarcinoma (n = 9), cribriform adenocarcinoma (n = 10), tumor with indeterminate features (n = 14), and tumor with predominant papillary pattern (n = 4)