Fig. 1: Representative images of HE staining, IHC and FISH for cases in ISH group 2. | Modern Pathology

Fig. 1: Representative images of HE staining, IHC and FISH for cases in ISH group 2.

From: A clinicopathological study of 30 breast cancer cases with a HER2/CEP17 ratio of ≥2.0 but an average HER2 copy number of <4.0 signals per cell

Fig. 1

a HE staining of a sample with invasive ductal carcinoma in ISH group 2. The tumor showed invasive growth. The tumor cells were arranged in adenoid and nest-like clusters. The tumor cells were atypical, with increased nuclear-plasma ratio and thicker chromatin. Nucleoli and mitotic images were visible (200×). b HE staining of a sample with invasive micopapillary carcinoma in ISH group 2. The tumor cells were in cleft like retraction spaces. The tumor cells were arranged in micropapillary pattern, with abundant eosinophilic cytoplasm (200×). c A representative image of IHC staining in ISH group 2 after a repeated test using the same tissue section applied for FISH assay. More than 10% of the tumor cells showed weak to moderate complete membrane staining. According to the 2018 ASCO/CAP guidelines, this case was interpreted as IHC 2+(200×). d A representative image of FISH after a second FISH reading in a case with IHC 2+. The result showed no change, with a HER2/CEP17 ratio of 2.74 compared with a HER2/CEP17 ratio of 2.71 from original test. The average HER2 copy number per cell was 3.47, while it was 1.27 for CEP17 (Red: HER2; Green: CEP17) (1000×).

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