Fig. 5: Inverse p53 immunohistochemical expression and HPV in situ hybridization results observed in HPV-associated neoplasias of the cervix and vulva.

Case 46 was an endocervical adenocarcinoma showing a markedly reduced (null-like) p53 staining and b HPV integration in nearly every tumor cell. Case 19 was a keratinizing squamous cell carcinoma of the vulva arising in a background of HSIL. In the invasive component c mid-epithelial classic p53 staining and d HPV viral integration confined to the single basal layer was observed; elsewhere e mid-epithelial central p53 staining and f HPV integration in the basal layer and variable parabasal layers was noted. In the associated HSIL, g mid-epithelial classic p53 staining was observed and h HPV integration was confined to the single basal layer.