Fig. 4: Emerging at 9 M, AD mice show a pronounced decrease in FC strength relative to WT mice.

a Similar to Fig. 2 we compare WT to AD groups at each timepoint. Positive t-values (red) indicate greater FC strength in AD vs. WT mice. Negative values (blue) indicate the opposite. Vertical histograms summarizing the number of positive/negative edges are displayed next to each matrix. b AD-like network for sum (left matrix) and intersect (right matrix), collapsed across 9 and 20 M timepoints (as indicated in a). AD-like network is also displayed as vertical histograms (right of each matrix), circle plot (middle) and node degree maps (bottom). Right: Fisher’s exact test (MATLAB) is used to assess (over/under) enrichment for edges within data-derived networks and the triple-network model: DMN, LCN, and SN, as well as their inter-network pairs (corrected, permutation test ×1000 iterations). Negative (−ve) edges are shown in blue (for AD < WT). No positive edges survive. Real data results are indicated with a grey vertical line and a black triangle. Null distribution is color-coded according to the network investigated (see Fig. 1a). A left-shift from the null distribution indicates under enrichment, a right-shift from the null distribution indicates over enrichment in the networks analyzed. Histograms indicate results from the sum. Significance is displayed as * for p <0.05, ** for p <0.01 and *** for p <0.001. ENT entorhinal, VIS visual, HF hippocampal formation, RSP retrosplenial, AUD auditory, TH thalamus, Tea temporal association area, SS somatosensory, ACB nucleus accumbens, MO motor, MB midbrain. Triple network color-coding: default-mode network (DMN) in green, salience network (SN) in pink, lateral-cortical network (LCN) in orange; regions in common across all three networks (light blue), regions shared by DMN and LCN (light purple) are also shown.