Fig. 4: tRNAGlyGCC overexpression in mouse increases the expression of protein with high glycine GGC codon content, REEP1. | Molecular Psychiatry

Fig. 4: tRNAGlyGCC overexpression in mouse increases the expression of protein with high glycine GGC codon content, REEP1.

From: Altered tRNA expression profile associated with codon-specific proteomic changes in the suicide brain

Fig. 4

a Mouse model of tRNAGlyGCC overexpression in which mice harbor ~27 copies of a transgene expressing two tRNA-Gly-GCC genes, leading to an additional ~54 copies of tRNA-Gly-GCC (tRNAGly-high) compared to wild-type controls lacking the transgene. b Taqman copy number assay demonstrated a significant increase in transgene copy number in tRNAGly-high mice compared to WT control mice (n = 6 tRNAGly-high/6 WT; unpaired t-test, t(10) = 18.96, p < 0.0001, two-tailed; ****p < 0.0001). c Western blot for REEP1 protein expression in tRNAGly-high cortex compared to wild-type controls and using actin as a loading control (n = 6 tRNAGly-high/6 WT; unpaired t-test, t(10) = 2.492, p = 0.0319, two-tailed; *p < 0.05).

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