Fig. 6: The behavioral analysis found social impairments but not anxiety-like behavior or compulsive-obsessive behavior in MDZ-exposed animals. | Molecular Psychiatry

Fig. 6: The behavioral analysis found social impairments but not anxiety-like behavior or compulsive-obsessive behavior in MDZ-exposed animals.

From: Comprehensive characterization of chronic midazolam exposure on neonates and long-term neurodevelopment

Fig. 6

A Sociability test revealed reductions of P28 MDZ animals in the total number of entries and contacts to the Naïve chamber but not at other time points. In addition, P28 MDZ also showed an overall reduction in entering the Toy chamber but did not differ in the number of contacts. Interestingly, P28 MDZ displayed a significantly lower number of touches per entry in the toy chamber (*p < 0.05, n = 37–39/group/timepoint), not the Naïve chamber. B The Social Novelty Preference test displayed no significant difference in time spent, number of entries, and contacts in Naïve and cagemate chambers in each condition and time point. However, P28 MDZ displayed a significant increase in the touches per entry to the cagemate chamber (*p < 0.05, n = 37–39/group/timepoint). C Marble burying assay analysis revealed no significant difference between MDZ and Saline at all time points (n = 32–34/group/timepoint). Repeated measures Two-way ANOVA with Sidak’s correction was used to determine the significant difference as data were represented as mean ± SEM. Data points for all panels and details on sample size, and statistical analyses are provided in Source Data and Statistical Supplemental data files.

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