Fig. 3: L. plantarum and NaB, restored gut function and behavior defects in MIA offspring. | Molecular Psychiatry

Fig. 3: L. plantarum and NaB, restored gut function and behavior defects in MIA offspring.

From: Probiotics derived sodium benzoate improves social behavior of offspring exposed in the maternal immune activation through regulation of histone lysine benzoylation in astrocytes

Fig. 3

(A) L. plantarum supernatant and sodium benzoate standard solution could react with ferric chloride to generate the ochre precipitation, but the MRS medium blank failed to. (B) The concentration fold change of NaB in the MRS medium blank and L. plantarum supernatants. n = 5 biological replicates /group. (C) The metabolic network map of NaB, red arrows represent the enzymes that correlated with the behavioral scales. (D) NaB catabolism enzyme EC1.17.1.4 was positively associated with the ABC scale score. (E) The statistical analysis of the FITC intensity in the serum. n = 4 mice/group. (F) Representative spectrum of NaB in the brain tissue. (G) NaB concentration in the brain of the offsprings was detected by targeted metabolomics. n = 4 mice /group. (H) The statistical analysis of the time that the offsprings interacted with the object and the stranger mouse. n = 9-10 mice/group. (I) The statistical analysis of the time that the offsprings interacted with the object and the stranger mouse. n = 9-10 mice/group. (J) The score of offsprings during the marble-burying test. n = 12 mice/group. (K) Representative immunofluorescence images of c-Fos expression in S1DZ. Scale bar: 50 µm. (L) Statistical analysis of c-Fos expression in S1DZ. n = 4 mice/group. (M) Representative immunofluorescence image of PSD95 expression in the hippocampal CA3 region. Scale bar: 20 µm. (N) The statistical analysis of PSD95 expression in the hippocampal CA3 region. n = 4 mice/group. (O) Representative immunofluorescence image of GFAP expression in the hippocampal CA1 region. Scale bar: 50 µm, 20 µm, 8 µm. (P) Statistical analysis of GFAP expression in the hippocampal CA1 region. n = 5 mice/group. Data were presented as mean ± SEM, and statistical significance was determined by Unpaired t-test (B, G), Tukey’s multiple comparisons test (E, J, L, N, and P) and Sidak’s multiple comparisons test (H, I), significant differences were classified as follows: ns p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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