Fig. 5: L. plantarum and NaB modulated the expression of Cxcl16 in hippocampal astrocytes to affect partial defects in social behavior in ASD mice. | Molecular Psychiatry

Fig. 5: L. plantarum and NaB modulated the expression of Cxcl16 in hippocampal astrocytes to affect partial defects in social behavior in ASD mice.

From: Probiotics derived sodium benzoate improves social behavior of offspring exposed in the maternal immune activation through regulation of histone lysine benzoylation in astrocytes

Fig. 5

(A) Schematic of viral infection and optical fiber implantation. (B-C) Inject AAV-GFaABC1D-mCherry-5’miR-30a-shRNA1(Cxcl16)-3’miR-30a-WPREs into hippocampal astrocytes to reduce CXCL16 expression, and then compare social behavior of both groups. (B) Left: Statistical analysis of the time that mice interacted with the object and the stranger mouse1. n = 14–17 mice/group. Right: Representative tracks of the mice moved in the three-chamber. (C) Left: Statistical analysis of the time that mice interacted with the mouse1 and the mouse2. n = 14–17 mice/group. Right: Representative tracks of the mice moved in the three-chamber. (D-E) Inject rAAV-GFaABC1D-Cxcl16-P2A-mCherry-WPRE-SV40pA to upregulate Cxcl16 expression in the hippocampal astrocytes of MIA offspring, and then compare social behavior between groups. (D) Left: Statistical analysis of the time that mice interacted with the object and the stranger mouse1. n = 16–17 mice/group. Right: Representative tracks of the mice moved in the three-chamber. (E) Left: Statistical analysis of the time that mice interacted with familiar and novel mice. n = 16–17 mice/group. Right: Representative tracks of the mice moved in the three-chamber. (F-I) Inject rAAV-hSyn-GCaMp6s-WPRE-hGHpA to reveal Ca2+ signaling between neuronal activity. (F) Top: The heatmap illustrates the Ca2+ signals of the PIC offsprings when interacting with the object in the sociability stage. Bottom: The peri-event plot of the mean Ca2+ signals corresponding to the heatmaps (F top). n = 8 mice/group. (G) The same as F but for the stranger mouse1 in the sociability stage. n = 8 mice/group. (H) The same as (G) but in the social novelty stage. n = 8 mice/group. (I) The same as (H) but for the stranger mouse2. n = 8 mice/group. (J-M) The same as (F-I) but for PIC+ Cxcl16 experimental mice. n = 8 mice/group. (N-Q) The same as (F-I) but for wide-type (WT) experimental mice. n = 8 mice/group. Data were presented as mean ± SEM, and statistical significance was determined by Sidak’s multiple comparisons test (B-E), significant differences were classified as follows: ns p > 0.05, *p < 0.05, ***p < 0.001, ****p < 0.0001.

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