Fig. 4
From: C3a is required for ILC2 function in allergic airway inflammation
C3a regulates ILC-T-cell cross-talk. 1 × 104 Flow-sorted lung Lin- (CD11b, CD11c, Gr1, B220, CD19, TCRb, TCRgd, CD49b, CD4, CD8, FcER1) ICOS+IL-33R+ ILC2 from wildtype and C3ar1−/− mice were co-cultured with 1 × 104 CFSE-labeled OVA-transgenic (D011.10Tg) lymph node CD4+ T cells in the presence of 1 µg/ml OVA 323–339 peptide for 5 days. a Representative flow plot showing pre- and post-sort purity of sorted lung ILC2. b CFSE-labeled DO11.10Tg T cells proliferation. c Total T-cell numbers (CD3+CD4+), d IL-2 production, e frequency IL-13+CD4+ cells, and supernatant levels of f IL-13 g GM-CSF, and h IFNγ production. Data are means + SEM, representative of two independent experiments. All flow cytometry gating is based on FMO controls and, non-CFSE-labeled T cells. *p < 0.05, ***p < 0.001
