Fig. 2

Pkm2^−/− mice are more susceptible to DSS-induced colitis compared to fl/fl mice. a Schema illustrating generation of intestinal-specific Pkm2^−/− mice. b Representative PCR, immunofluorescence, and blot images of Pkm2 and Pkm1 protein in IECs isolated from fl/fl and Pkm2^−/− mice. c Percentage reduction in bodyweight in fl/fl and Pkm2^−/− mice treated with 2.5% DSS for 7 days (fl/fl, n = 6; Pkm2^−/−, n = 9. d Clinical disease activity index of mice subjected to DSS-induced colitis for 7 days (fl/fl, n = 6; Pkm2^−/−, n = 9). e Kaplan–Meier survival analysis of fl/fl and Pkm2^−/− mice (each 12 total) following DSS treatment. f Representative pictures of colons on day 0, 3, 7 and colon length of fl/fl and Pkm2^−/− mice on days 0, 3, and 7 post DSS treatment (day 0, n = 5; days 3 and 7, n = 6). g H&E staining of colon sections of fl/fl and Pkm2^−/− mice on days 3 and 7 post DSS treatment. Bars, 100 μm. Histological examination and disease scores of respective groups were shown on days 3 and day 7. All p values are determined by the Student’s t test and two-way ANOVA analysis. ***p < 0.001; **p < 0.01; *p < 0.05