Fig. 6 | Mucosal Immunology

Fig. 6

From: Intestinal epithelial PKM2 serves as a safeguard against experimental colitis via activating β-catenin signaling

Fig. 6

Effect of Pkm2 on wound healing of IEC-6 monolayers under the stimulation of Wnt3a. a Treatment with Pkm2 siRNA and Pkm1 siRNA analyzed the healing process of wounded IEC-6 monolayers (upper panel) and cell proliferation (lower panel). b Overexpression of Pkm2 and Pkm1 in IEC-6 cells via transfecting cells with plasmid analyzed the wound closure of IEC-6 monolayers (upper panel) and cell proliferation (lower panel) compared to empty vector transfection. c Effect of Pkm2 inhibitor and activator on IEC-6 cell wound healing (upper panel) and cell proliferation (lower panel). d Overexpression of Pkm2 plasmid in IEC-6 cells via transfecting cells under the β-catenin siRNA analyzed the wound closure of IEC-6 monolayers (upper panel) and cell proliferation (lower panel) compared to empty vector transfection. Data are presented as the mean ± SEM from three independent experiments and p values are determined by the Student’s t test. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05; n.s. not significant

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