Fig. 7 | Mucosal Immunology

Fig. 7

From: Intestinal epithelial PKM2 serves as a safeguard against experimental colitis via activating β-catenin signaling

Fig. 7

Increase of colonic epithelial Pkm2 level via delivery of Pkm2-expressing plasmid attenuated mouse DSS-induced colitis. a Experimental design. b Successful delivery of mCherry Pkm2-expressing plasmid/PEI to mouse IECs detected by mCherry fluorescence. c WB analysis of Pkm2 level in mouse IECs with or without administration of Pkm2-expressing plasmid/PEI. d–g Delivery of Pkm2-expressing plasmid/PEI mitigated mouse colitic disease condition caused by DSS treatment: d percentage reduction in bodyweight; e disease index; f colon length; g colon section H&E staining and histological analysis. For all experiments, control (CTL) plasmid group and Pkm2 plasmid group consisted of nine and eight mice, respectively. Data are presented as the mean ± SEM. P values are determined by the Student’s t test and two-way ANOVA. **p < 0.01; *p < 0.05

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