Fig. 2: β-Catenin RIP enriches with Wnt signalling mRNAs. | Oncogene

Fig. 2: β-Catenin RIP enriches with Wnt signalling mRNAs.

From: β-Catenin interacts with canonical RBPs including MSI2 to associate with a Wnt signalling mRNA network in myeloid leukaemia cells

Fig. 2

Volcano plots showing the fold change in mRNA abundance detected within β-catenin RIP performed from DMSO (basal Wnt signalling) versus CHIR99021 (activated Wnt signalling) treated A K562 or B HEL cells (n = 3). Red dots represent enriched (Padj < 0.05) mRNAs whilst blue dots highlight Wnt signalling mRNAs and black dots highlight metabolic mRNAs. C Venn diagram illustrating the unique and shared mRNAs partners identified from β-catenin RIP performed from CHIR99021 versus DMSO treated K562 or HEL cells. D Gene ontology (GO) analysis using the human Molecular Signatures Database (Elsevier Pathway Collection) via Enrichr for pathways represented amongst the most significantly and commonly enriched mRNAs (Padj < 0.05), obtained in β-catenin RIP from K562 and HEL cells ±CHIR99021 with adjusted −Log10 P values annotated. Summary graphs showing the fold enrichment of selected Wnt signalling mRNAs isolated from IgG or β-catenin RIP-RT-qPCR performed in E K562 and F HEL cells. Data represents mean ± 1 s.d (n = 3). Statistical analysis is denoted by *p < 0.05 and **p < 0.01 as deduced from a one-sample t-test.

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