Fig. 8: Further validation by scRNA-seq and ST-seq.
From: SIX1 transmits signals for breast cancer progression via the ZEB1/IL6/STAT3 signaling axis
A UMAP representation of eight distinct cell subtypes in BC1 from scRNA-seq data. B Marker genes of eight cell subtypes. C Hierarchical heatmap of CNV scores of eight cell subtypes estimated by InferCNV. UMAP representation of CNV scores (D) and CNV status (E) in BC1. UMAP representation of SIX1 (F), STAT3 (G), and MYC (H) expression in BC1. Violin plot showing the expression of SIX1 (I), STAT3 (J), and MYC (K) in SIX1- and SIX1+ cancer cells in BC1. L ST-seq data showing expression of SIX1 and STAT3 in BC1. M Selective display of GO: BP enrichment results of SIX1+ and SIX1- cancer cells’ differential genes related to EMT and CSCs regulation in BC1. N KEGG enrichment results of SIX1+ and SIX1- cancer cells’ differential genes in BC1. O ST-seq data showing localization of indicated subgroups in BC1. P Violin plot showing the expression of IL6R of indicated subgroups in BC1 from ST-seq data. Q Ripley’s function showing the distribution of indicated subgroups in BC1 from ST-seq data. R Co-occurrence score among indicated subgroups in BC1 at increasing distance threshold across the tissue from ST-seq data. S Identification of breast cancer cell compartments using NMF is depicted in a dot plot, illustrating the estimated NMF weights of different cell types (rows) across NMF components (columns) in BC1 from ST-seq data.
