Fig. 5: LAMP5 promotes the nuclear import of IRF4 regulated by KPNA2. | Oncogene

Fig. 5: LAMP5 promotes the nuclear import of IRF4 regulated by KPNA2.

From: LAMP5 modulates IRF4 stability and nuclear transport: a critical mechanism in myeloma progression and therapy

Fig. 5

A Analysis of immunoprecipitates of HA-tagged LAMP5 in IM-9 cells with mass spectrometry. The proteins identified are indicated. B, C Co-immunoprecipitation of KPNA2 with IRF4 or LAMP5 in HEK293T cells co-transfected with HA-KPNA2 and IRF4 or LAMP5 plasmids. D Pull-down of HA-KPNA2 with IRF4 in HEK293T cells. E Pull-down of HA-KPNA2 with LAMP5 in HEK293T cells. F Pull-down of KPNA2 with different truncations of IRF4 (ΔDBD and ΔIAD) in HEK293T cells. G Co-immunoprecipitation of KPNA2 with IRF4 in ARP-1 or IM-9 cells. H Co-immunoprecipitation of KPNA2 with IRF4 in ARP-1 or IM-9 cells (shCtrl or shLAMP5). I The levels of IRF4 protein in the cytoplasm and nucleus of shCtrl or shLAMP5 myeloma cells. J, K Immunofluorescent staining of ARP-1 or IM-9 cells (shCtrl or shLAMP5) with DAPI and antibodies against IRF4 or KPNA2. Scale bar, 6 μm. L Immunofluorescent staining of tumor tissues in Fig. 2H with DAPI and antibodies against KPNA2 and IRF4. Scale bar, 20 μm. B-I are representative of two independent experiments. J–L are representative of three independent experiments.

Back to article page