Fig. 6: c-MYC positively feedbacks to activate the transcription of LAMP5. | Oncogene

Fig. 6: c-MYC positively feedbacks to activate the transcription of LAMP5.

From: LAMP5 modulates IRF4 stability and nuclear transport: a critical mechanism in myeloma progression and therapy

Fig. 6

A–C The expression of c-MYC or LAMP5 in ARP-1 or IM-9 cells (shCtrl or shLAMP5). Nontargeted shRNA (shCtrl) served as control. D ChIP assay showing c-MYC enrichment in the LAMP5 promoter in myeloma cell lines (ARP-1 or IM-9). E, F Schematic of the LAMP5 promoter luciferase reporter. Solid boxes: promoter region of LAMP5; red crosses: mutations of nucleotides. The luciferase activity of LAMP5-Full constructs was set at 1. G Depiction of LAMP5-mediated signaling pathways in myeloma cells. A, B, D, and F are representative of three independent experiments. C is representative of two independent experiments. Data are averages ±SD. A, B, D P values were determined by unpaired two-tailed t test.

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