Fig. 7: CRT0105950 inhibits LIMK2/MST4/NPM1 signaling pathway and tumor cell growth in vitro and in vivo. | Oncogene

Fig. 7: CRT0105950 inhibits LIMK2/MST4/NPM1 signaling pathway and tumor cell growth in vitro and in vivo.

From: LIMK2 promotes centrosome clustering and cancer progression by activating MST4-mediated phosphorylation of NPM1

Fig. 7

A Multipolar spindle formation in CRT0105950-treated cells as shown by the Pericentrin (orange), Centrin 1 (green), α-Tubulin (red), and DNA (DAPI, blue). Scale bar is 5 µm. Quantification of mitotic cells with multipolar spindles from indicated cells. Each experiment involves more than 80 metaphase cells (n = 3, independent experiments). B Flow cytometry analysis Annexin V/PI staining in cells following 48 h treatment with CRT0105950 (n = 3, independent experiments). C Western blot analysis of mitosis marker and apoptosis marker in indicated cells following 48 h treatment with CRT0105950. D LIMK2/MST4/NPM1 signaling pathway related protein expression levels were examined by western blot in cells after CRT0105950 treatment. E Representative images and tumor growth curve of CDX in each treatment group (n = 8/group). All data were shown as means ± SD and were analyzed with the two-tailed Student’s t test. *p < 0.05, **p < 0.01, ***p < 0.001.

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