Fig. 2: NUCKS1 enhances the invasion and metastasis capacities of CRC cells via HDAC2/p-AKT.
From: NUCKS1 promotes invasion and metastasis of colorectal cancer by stabilizing HDAC2 and activating AKT
a Heat map showing 28 differentially expressed proteins in NUCKS1-silenced HCT116 cells compared to control cells. b Detection of HDAC2 and phosphorylated AKT expression levels in NUCKS1-silenced and NUCKS1-overexpressing cell lines using Western blotting. c, d Western blotting assay determining HDAC2 and phosphorylated AKT protein expression levels in HCT116 and SW480 cells under various conditions. e, f Transwell assays measuring migration and invasion abilities of HCT116 and SW480 cells under different conditions. Scale bar = 100 μm. g Changes in phosphorylated AKT levels in SW480 cells overexpressing HDAC2 treated with LY294002 inhibitor (10 µM). h Changes in cell invasion and migration in SW480 cells overexpressing HDAC2 treated with LY294002 inhibitor (10 µM). Scale bar = 100 μm. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.
