Fig. 4: NUCKS1 interacts with and stabilizes HDAC2.
From: NUCKS1 promotes invasion and metastasis of colorectal cancer by stabilizing HDAC2 and activating AKT
a, b Detection of endogenous or exogenous NUCKS1 binding to HDAC2 using Co-IP and Western blotting. c, d Immunofluorescence showing the colocalization of NUCKS1 and HDAC2 following treatment with shRNAs for NUCKS1 or NUCKS1 overexpression. Scale bar = 20 μm. Intensity profiles of NUCKS1 and HDAC2 along the white line are plotted on the right. e, f Statistical results of mean fluorescence intensity (MFI) of NUCKS1 and HDAC2. g Detection of HDAC2 mRNA levels in NUCKS1-silenced HCT116 cells and NUCKS1-overexpressing SW480 cells. h Detection of HDAC2 protein levels in NUCKS1-silenced HCT116 cells and control cells after treatment with the protein synthesis inhibitor cycloheximide (CHX) for 8, 24, and 48 hours). ns represents no statistical difference, **p < 0.01 and ****p < 0.0001.
