Fig. 4: LDAH knockdown increases TAG hydrolysis in ovarian cancer cells. | Oncogenesis

Fig. 4: LDAH knockdown increases TAG hydrolysis in ovarian cancer cells.

From: Lipid droplet-associated hydrolase (LDAH) knockdown enhances TAG hydrolysis and promotes ovarian cancer progression and chemoresistance

Fig. 4

A BODIPY 493/503 staining (green) of lipid droplets in scrambled, LDAH knockdown (shLDAH), and LDAH overexpressing (OE) ES2 cancer cells. Blue, DAPI. Scale bars, 10 µm. Quantification of BODIPY signals is shown on the right. One-way ANOVA followed by Tukey’s multiple comparison test. B TAG levels (mg/dl) in scrambled, LDAH knockdown (shLDAH), and LDAH overexpressing (OE) ES2 cancer cells. One-way ANOVA followed by Tukey’s multiple comparison test. Western blot of CPT1A in scrambled control and LDAH knockdown ES2 (C) and A2780 (D) cancer cells with or without cisplatin treatment. The quantification of band intensity normalized to GAPDH is shown below. One-way ANOVA followed by Tukey’s multiple comparison test. Western blot of p-NF-kB and total NF-kB in scrambled control and LDAH knockdown ES2 (E) and A2780 (F) cancer cells with or without cisplatin treatment. The quantification of band intensity normalized to GAPDH is shown below. One-way ANOVA followed by Tukey’s multiple comparison test. Error bars, SD. *, p < 0.05, **, p < 0.01, ***, p < 0.001, ****, p < 0.0001.

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