Fig. 3: Knockout of SLC7A5 in LUSC cells. | Oncogenesis

Fig. 3: Knockout of SLC7A5 in LUSC cells.

From: Targeting SLC7A5 in lung squamous cell carcinoma: implications for cancer metabolism shift and boron neutron capture therapy resistance

Fig. 3

A Immunoblotting analysis of SLC7A5 expression in wild-type H2170 (WT) and its SLC7A5 knockout cells (KO1 and KO2), which harbor differential deletion/mutation regions in the SLC7A5 coding region. B Clonogenic assay assessing colony growth of H2170 and its SLC7A5 knockout cells (KO1 and KO2). Colonies were stained with crystal violet (bottom) and quantified by ImageJ software (top). ns, p > 0.05. C Annexin V flow cytometric analysis assessing the apoptotic rate in H2170 and its SLC7A5 knockout cells (KO1 and KO2). ns, p > 0.05. D Flow cytometric analysis of the cell-cycle distribution in H2170 and its SLC7A5 knockout cells (KO1 and KO2). ns, p > 0.05. E RNA-seq analysis assessing the indicated amino acid transporter gene (SLC3A2, SLC1A5, SLC1A4, and SLC38A5) expression in H2170 and its SLC7A5 knockout cells (KO1 and KO2). F RT-qPCR analysis assessing amino acid transporter gene (SLC3A2, SLC1A5, SLC1A4, and SLC38A5) expression in H2170 and its SLC7A5 knockout cells (KO1 and KO2). **p < 0.01; ***p < 0.001. G Scatter plot analysis assessing the correlation between indicated amino acid transporter genes SLC7A5, SLC3A2, SLC1A5, and SLC1A4 expression in lung squamous cell carcinoma from the TCGA database. ***p < 0.001.

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