Fig. 4: Identification of specific glycoRNAs enriched in U87 and LN229 cells.
From: GlycoRNAs are abundant in glioma and involved in glioma cell proliferation
A Scatterplot showing the enriched glycoRNAs in the U87 and LN229 groups. The vertical axis: U87, log2FC. The horizontal axis: LN229, log2FC. The size and color of the scatters were used to represent the -log10(Pvalue) of each RNA in the LN229 and U87 groups, respectively. B qRT-PCR analysis showing the expression levels of glycoRNA U1, U2, U4, and Y5 in glioma cells including U87 and LN229, and non-glioma cells, such as Hela, MCF7 and HEK-293T. 0 h represents the control group that did not undergo Ac4ManNAz labeling but went through a series of extraction and purification processes of glycoRNAs. While the 24 h group represents the products of cells after the entire process of Ac4ManNAz labeling and extraction and purification of glycoRNAs, i.e. glycoRNAs. *p < 0.05; **p < 0.01; ***p < 0.001; ns p > 0.05.
