Fig. 7: glycoRNA depletion experiments.
From: GlycoRNAs are abundant in glioma and involved in glioma cell proliferation
A Flow plot showing the procedures of glycoRNA depletion experiments. B Fluorescence imaging showing the distribution of FITC-labeled RNaseA following 30 min of incubation. C, D RNA blotting showing the biotin signals in RNA samples isolated from treated U87 and LN229 cells. Treatment conditions were: C 30-min exposure to RNaseA alone or RNaseA plus RNasin; D treatment as in C followed by 12-h culture in complete medium. strep: RNA-transferred membrane was treated with anti-biotin streptavitin-HRP and imaged. sybr: before RNA transfer to the membrane, the RNA in the gel was stained with SYBR Gold and imaged. CCK-8 analysis showing the cell viability of U87 (E) and LN229 (F) cells treated with either RNaseA alone or RNaseA plus RNasin for 30 min, followed by 12-h culture in complete medium. Cell adhesion analysis on U87 (G) and LN229 (H) cells treated with either RNaseA alone or RNaseA plus RNasin for 30 min, followed by 12-h culture in complete medium. ****p < 0.0001; ns: p > 0.05.
