Fig. 4: Effect of Na-L-lactate, pH, glucose and conditioned medium on EO771 outgrowth in co-cultures. | Oncogenesis

Fig. 4: Effect of Na-L-lactate, pH, glucose and conditioned medium on EO771 outgrowth in co-cultures.

From: Transcriptomic profiling of co-cultured cancer-host cells identifies hypoxia as a driver of the skeletal muscle cell’s anti-proliferative effect on cancer cells

Fig. 4

A Schematic representation of co-cultures. B EO771 (4000 cells per well in 96-well plate) outgrowth in co-culture with C2C12 myotubes (C2C12 + EO771) or MLg cells (MLg + EO771) with added Na-L-lactate (n = 12), C Altered medium pH (n = 10–12), or D Added Na-L-lactate and altered medium pH (n = 9–10). All pH adjustments and measurements were done at room temperature. For (B–D), representative pictures can be found in Fig. S9. E Outgrowth of EO771 cells cultured for 2 days in medium with different glucose concentrations (n = 24–27). F Outgrowth of EO771 cells co-cultured for 2 days with C2C12 myotubes (C2C12 + EO771) or MLg cells (MLg + EO771) in medium with 5.5 mM or 25 mM glucose (n = 12). G Outgrowth of EO771 cells cultured in conditioned medium from C2C12 myotubes or MLg cells (n = 21). The EO771 area represents the percentage of fluorescent cells normalized to day 0 (D0). Data are mean ± SEM from two independent experiments. Comparison between groups was done using two-way ANOVA with Šidák (only two groups) or Tukey’s (more than two groups) comparisons. *P < 0.05; **P < 0.01; ****P < 0.0001.

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